Circular probe amplification (CPA) using energy-transfer primers
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United States of America Patent
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Jul 12, 2011
Grant Date -
Sep 20, 2007
app pub date -
Dec 12, 2006
filing date -
Feb 27, 2001
priority date (Note) -
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Abstract
The present invention provides methods and kits for the rapid exponential amplification of nucleic acid molecules using a padlock probe. The present invention improves upon the existing methods for amplifying padlock probes by eliminating or delaying the appearance of artifact products that cause false positive results, and also increase the sensitivity and speed of the assay. Further provided are nucleic acid amplification primers containing non-informative base analogs.
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