Method of determining the mutational load of a gene library obtained by random mutagenesis of a particular gene and means for implementing same
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United States of America Patent
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Jul 19, 2011
Grant Date -
Jun 12, 2008
app pub date -
Jun 7, 2005
filing date -
Jun 7, 2004
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Abstract
The invention relates to methods of determining the mutational load of a gene bank obtained by random mutagenesis of a gene of interest by preparing a chart linking the mutational load of a gene bank obtained by random mutagenesis of a model gene with the fraction of mutated model genes observed in the bank; performing, in parallel, random mutagenesis of the model gene used for preparing the chart and the gene of interest to obtain the corresponding mutated gene banks; determining the mutational load of the gene bank obtained using the model gene on the basis of the chart plotted; and applying a correction factor to the mutational load of the mutated model gene bank to determine the mutational load in the gene bank of the mutated genes of interest.
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