Methods and apparatus for amplification of DNA using sonic energy
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United States of America Patent
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Aug 9, 2011
Grant Date -
Jun 18, 2009
app pub date -
Feb 18, 2009
filing date -
May 13, 2005
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Abstract
Apparatus and methods for amplification of DNA are provided that use sonic energy in place of conventional thermocyclers. In one embodiment, sonic energy is applied to a PCR cocktail to effect dissociation of double stranded DNA into single strands of DNA. A quiescence stage, where no sonic energy is applied, results in amplification of DNA. Repetition of the cycles of application of sonic energy and cessation of application of sonic energy results in DNA amplification.
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