Method for sequencing a polynucleotide template
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United States of America Patent
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Sep 13, 2011
Grant Date -
Jul 16, 2009
app pub date -
Jul 20, 2006
filing date -
Jul 20, 2005
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Abstract
The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which permit the sequential determination of nucleotide sequences in two distinct and separate regions on complementary strands of the double-stranded polynucleotide template. The two regions for sequence determination may or may not be complementary to each other.
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