Isothermal nucleic acid amplification methods and compositions
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United States of America Patent
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Oct 11, 2011
Grant Date -
Aug 13, 2009
app pub date -
Feb 12, 2009
filing date -
Feb 12, 2008
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Abstract
Methods and compositions are provided related to the amplification of target polynucleotide sequences as well as total RNA and total DNA amplification. In some embodiments, the methods and compositions also allow for the immobilization and capture of target polynucleotides with defined 3′ and or 5′ sequences to solid surfaces. The polynucleotides attached to the solid surfaces can be amplified or eluted for downstream processing. In some cases, nucleotides attached to solid surfaces can be used for high throughput sequencing of nucleotide sequences related to target DNA or target RNA.
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