Method for isolating both free and protein association DNA
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United States of America Patent
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Nov 22, 2011
Grant Date -
Mar 11, 2010
app pub date -
Jan 15, 2007
filing date -
Jan 16, 2006
priority date (Note) -
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Abstract
A method is disclosed for isolating both free and protein-associated DNA from bodily fluids, such as urine, saliva, serum, tears, sweat, cerebral spinal fluid, and plasma. The method comprises as a first step concentrating and isolating both the free DNA and the proteins present in the bodily fluid. The proteins are then digested in order to release the formerly protein-associated DNA from the isolated proteins. Lastly, the free and formerly protein-associated DNA can be isolated and purified.
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