Method for the high throughput screening of transposon tagging populations and massive parallel sequence identification of insertion sites
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United States of America Patent
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Dec 6, 2011
Grant Date -
Aug 20, 2009
app pub date -
Nov 8, 2006
filing date -
Nov 14, 2005
priority date (Note) -
Expired
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Abstract
A method for the identification of a gene in a transposon population is provided. The method comprises isolating genomic DNA, optionally pooling the DNA, restricting the DNA in the pools using an enzyme, ligating adaptors, amplifying the adaptor-ligated fragments with primers one of which is a primer complementary to a border of a transposon sequence, sequencing the fragments using high throughput sequencing, aligning the fragments with known sequences in a database and thereby identifying gene candidates.
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