US Patent No: 8,119,353
Number of patents in Portfolio can not be more than 2000
Rapid one-step reverse transcriptase PCR
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Feb 21, 2012
Issued date -
Apr 3, 2008
filing date -
12/061,700
serial no -
In Force
status
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Abstract
The present invention is directed to a method for performing a one-step RT-PCR for amplifying a target RNA comprising the steps (i) providing a sample which is supposed to contain said target RNA (ii) adding a reaction mixture comprising all reagents necessary to reverse transcribe said target RNA into cDNA and amplify at least a portion of said cDNA (iii) incubating said sample for a time interval of 0 seconds to 40 seconds at a temperature between 20° C. and 65° C., and (iv) subjecting said sample to multiple cycles of a thermocycling protocol wherein the temperature of said sample is varied between at least a first temperature between 37° C. and 72° C. and a second temperature between 85° C. and 100° C.
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First Claim
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International Classification(s)
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Cited Art
| Patent Info | (Count) | # Cites | Year |
|---|---|---|---|
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| 5,210,015 Homogeneous assay system using the nuclease activity of a nucleic acid polymerase | 389 | 1990 | |
| 5,487,972 Nucleic acid detection by the 5'-3'exonuclease activity of polymerases acting on adjacently hybridized oligonucleotides | 174 | 1993 | |
| 5,804,375 Reaction mixtures for detection of target nucleic acids | 110 | 1995 | |
| 5,561,058 Methods for coupled high temperatures reverse transcription and polymerase chain reactions | 52 | 1995 | |
| 5,624,833 Purified thermostable nucleic acid polymerase enzyme from Thermotoga maritima | 45 | 1995 | |
| 5,674,738 DNA encoding thermostable nucleic acid polymerase enzyme from thermus species Z05 | 76 | 1995 | |
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| 2003/0108,898 Method for detection, quantitation and breakpoint cluster region determination of fusion transcripts | 1 | 2002 | |
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| 5,876,924 Nucleic acid amplification method hybridization signal amplification method (HSAM) | 94 | 1996 | |
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| 5,118,801 Nucleic acid process containing improved molecular switch | 154 | 1988 | |
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| 5,538,848 Method for detecting nucleic acid amplification using self-quenching fluorescence probe | 297 | 1994 | |
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| 6,174,670 Monitoring amplification of DNA during PCR | 157 | 1997 | |
Patent Citation Ranking
Maintenance Fees
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| 3.5 Year Payment | $1600.00 | $800.00 | $400.00 | Aug 21, 2015 |
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| 11.5 Year Payment | $7400.00 | $3700.00 | $1850.00 | Aug 21, 2023 |
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