US Patent No: 8,119,353

Number of patents in Portfolio can not be more than 2000

Rapid one-step reverse transcriptase PCR

ALSO PUBLISHED AS: 20080248535

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Abstract

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The present invention is directed to a method for performing a one-step RT-PCR for amplifying a target RNA comprising the steps (i) providing a sample which is supposed to contain said target RNA (ii) adding a reaction mixture comprising all reagents necessary to reverse transcribe said target RNA into cDNA and amplify at least a portion of said cDNA (iii) incubating said sample for a time interval of 0 seconds to 40 seconds at a temperature between 20° C. and 65° C., and (iv) subjecting said sample to multiple cycles of a thermocycling protocol wherein the temperature of said sample is varied between at least a first temperature between 37° C. and 72° C. and a second temperature between 85° C. and 100° C.

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Patent Owner(s)

Patent OwnerAddressTotal Patents
ROCHE DIAGNOSTICS OPERATIONS, INC.INDIANAPOLIS, IN1222

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Ankenbauer, Waltraud Penzberg, DE 36 34
Grepl, Ursula Grafelfing, DE 2 0
Haerteis, Rita Ried-Kochel a. S., DE 3 0

Cited Art Landscape

Patent Info (Count) # Cites Year
 
ROCHE MOLECULAR SYSTEMS, INC. (6)
5,210,015 Homogeneous assay system using the nuclease activity of a nucleic acid polymerase 425 1990
5,487,972 Nucleic acid detection by the 5'-3'exonuclease activity of polymerases acting on adjacently hybridized oligonucleotides 190 1993
5,804,375 Reaction mixtures for detection of target nucleic acids 125 1995
5,561,058 Methods for coupled high temperatures reverse transcription and polymerase chain reactions 57 1995
5,624,833 Purified thermostable nucleic acid polymerase enzyme from Thermotoga maritima 48 1995
5,674,738 DNA encoding thermostable nucleic acid polymerase enzyme from thermus species Z05 80 1995
 
GENZYME CORPORATION (1)
2003/0108,898 Method for detection, quantitation and breakpoint cluster region determination of fusion transcripts 1 2002
 
MOUNT SINAI SCHOOL OF MEDICINE (1)
5,876,924 Nucleic acid amplification method hybridization signal amplification method (HSAM) 108 1996
 
PUBLIC HEALTH RESEARCH INSTITUTE (1)
5,118,801 Nucleic acid process containing improved molecular switch 170 1988
 
The Perkin-Elmer Corporation (1)
5,538,848 Method for detecting nucleic acid amplification using self-quenching fluorescence probe 332 1994
 
UNIVERSITY OF UTAH (1)
6,174,670 Monitoring amplification of DNA during PCR 183 1997

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