Rapid one-step reverse transcriptase PCR

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United States of America Patent

PATENT NO 8119353
APP PUB NO 20080248535A1
SERIAL NO

12061700

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Abstract

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The present invention is directed to a method for performing a one-step RT-PCR for amplifying a target RNA comprising the steps (i) providing a sample which is supposed to contain said target RNA (ii) adding a reaction mixture comprising all reagents necessary to reverse transcribe said target RNA into cDNA and amplify at least a portion of said cDNA (iii) incubating said sample for a time interval of 0 seconds to 40 seconds at a temperature between 20° C. and 65° C., and (iv) subjecting said sample to multiple cycles of a thermocycling protocol wherein the temperature of said sample is varied between at least a first temperature between 37° C. and 72° C. and a second temperature between 85° C. and 100° C.

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Patent Owner(s)

Patent OwnerAddressTotal Patents
ROCHE DIAGNOSTICS OPERATIONS, INC.INDIANAPOLIS, IN497

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Ankenbauer, Waltraud Penzberg, DE 27 149
Grepl, Ursula Grafelfing, DE 1 1
Haerteis, Rita Ried-Kochel a. S., DE 2 1

Cited Art Landscape

Patent Info (Count) # Cites Year
 
UNIVERSITY OF UTAH (1)
6174670 Monitoring amplification of DNA during PCR 293 1997
 
GENZYME CORPORATION (1)
* 2003/0108,898 Method for detection, quantitation and breakpoint cluster region determination of fusion transcripts 1 2002
 
MOUNT SINAI SCHOOL OF MEDICINE (1)
* 5876924 Nucleic acid amplification method hybridization signal amplification method (HSAM) 131 1996
 
PERKIN-ELMER CORPORATION, THE (1)
5538848 Method for detecting nucleic acid amplification using self-quenching fluorescence probe 441 1994
 
PUBLIC HEALTH RESEARCH INSTITUTE (1)
5118801 Nucleic acid process containing improved molecular switch 204 1988
 
ROCHE MOLECULAR SYSTEMS, INC. (6)
5210015 Homogeneous assay system using the nuclease activity of a nucleic acid polymerase 552 1990
5487972 Nucleic acid detection by the 5'-3'exonuclease activity of polymerases acting on adjacently hybridized oligonucleotides 240 1993
5804375 Reaction mixtures for detection of target nucleic acids 170 1995
5561058 Methods for coupled high temperatures reverse transcription and polymerase chain reactions 66 1995
5624833 Purified thermostable nucleic acid polymerase enzyme from Thermotoga maritima 52 1995
5674738 DNA encoding thermostable nucleic acid polymerase enzyme from thermus species Z05 90 1995
* Cited By Examiner

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