System for multi color real time PCR
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United States of America Patent
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Mar 20, 2012
Grant Date -
Nov 30, 2006
app pub date -
Apr 1, 2004
filing date -
Apr 4, 2003
priority date (Note) -
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Abstract
The invention is directed to a system for performing multi-color real time PCT, comprising a flexible real time PCT instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixture which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.
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