Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
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United States of America Patent
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Mar 20, 2012
Grant Date -
Sep 2, 2004
app pub date -
Nov 21, 2003
filing date -
May 22, 2001
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Expired
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Abstract
The present invention provides methods, compositions and kits for using an RNA polymerase for making transcription products corresponding to a target sequence by obtaining single-stranded DNA transcription substrates that comprise a single-stranded promoter that is operably joined to a single-stranded target sequence. The invention has broad applicability for research, diagnostic and therapeutic applications, such as preparing cDNA corresponding to full-length mRNA, making sense or anti-sense probes, detecting gene- or organism-specific sequences, cloning, cell signaling or making RNA for use in RNAi.
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