Polyelectrolytic internal calibration system of a flow-through assay
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United States of America Patent
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Mar 20, 2012
Grant Date -
Mar 11, 2010
app pub date -
Nov 12, 2009
filing date -
Dec 24, 2001
priority date (Note) -
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Abstract
A flow-through assay for detecting the quantity of an analyte residing in a test sample is provided. The flow-through assay contains a porous membrane that is in fluid communication with probe conjugates that contain a specific binding member and a detectable probe. The porous membrane also defines a detection zone and a calibration zone. The calibration zone contains a polyelectrolyte substantially non-diffusively immobilized on the porous membrane. The polyelectrolyte is capable of generating a detectable calibration signal that can be readily compared (visually, quantitatively, and the like) to a detection signal to determine the amount of analyte in the test sample.
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