Compositions and method for storage of nucleic acid from bodily fluids
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United States of America Patent
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Apr 17, 2012
Grant Date -
May 14, 2009
app pub date -
Mar 14, 2006
filing date -
Mar 16, 2005
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Abstract
An aqueous composition comprising a denaturing agent, a chelator, a buffering agent and a protease for the extraction of nucleic acid from a sample of bodily fluid, such as saliva, such that the extracted nucleic acid is stable for at least fourteen days at room temperature and can be directly utilized in an amplification reaction without further processing. In particular, said composition comprises SDS, Cyclohexanediamine tetraacetate, Tris-HCl and proteinase K. A method and kit for the amplification of DNA directly from a bodily fluid, comprising said composition, is further provided.
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