Repair of nucleic acids for improved amplification
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United States of America Patent
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Apr 17, 2012
Grant Date -
Aug 10, 2006
app pub date -
Apr 11, 2006
filing date -
Oct 21, 2004
priority date (Note) -
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Abstract
Methods and compositions are provided for repairing a polynucleotide so that it can be copied with improved fidelity and/or yield in, for example, an amplification reaction. This involves the use of a reaction mixture that includes a ligase and a cofactor selected from NAD+ or ATP and incubating the polynucleotide with the reaction mixture in the absence of Endonuclease VI.
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