Method for a continuous rapid thermal cycle system
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United States of America Patent
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Apr 24, 2012
Grant Date -
Oct 21, 2010
app pub date -
Apr 6, 2009
filing date -
Oct 6, 2006
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Abstract
Disclosed herein is an efficient, high speed production scale synthesis method for high molecular weight organic substances, such as DNA. The invention includes a method of conducting a polymerase chain reaction which comprises transporting a liquid through polymeric tubing disposed through a first reaction cycle region and at least a second reaction cycle region, each of which regions comprises at least a first and a second temperature zone, the temperature in each zone of said at least second region being substantially identical to the corresponding first and second zones in said first region, wherein said liquid is an aqueous solution comprising polymerase chain reaction reactants and a surface absorbing polymer.
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