DNA polymerases
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United States of America Patent
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May 22, 2012
Grant Date -
Dec 24, 2009
app pub date -
Oct 9, 2007
filing date -
Oct 9, 2006
priority date (Note) -
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Abstract
The present invention relates to a thermophilic polymerase, wherein the DNA polymerase has an in-vitro primer extension rate that is >35 bases/second and faster relative to the primer extension rate of a DNA polymerase comprising amino acid sequences SEQ ID NO: 2 or 4, when measured under identical conditions in a DNA replication assay using primed single strand M13mp18 DNA and an incubation temperature of 60° C. The invention also relates to a vector comprising the polymerase, a host cell comprising the vector. The invention relates to a nucleic acid replication kit comprising the polymerase according to the invention.
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