Methods for quantifying microRNA precursors

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United States of America Patent

PATENT NO 8192938
APP PUB NO 20090123917A1
SERIAL NO

11816767

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Abstract

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The present invention is directed to methods, reagents, kits and compositions for detecting microRNA (miRNA) precursors in a biological sample. The methods uses gene-specific primers and reverse transciptase to convert the primary miRNA precursors (pri-miRNA) and pre-miRNA precursors (pre-miRNAs) to cDNA. The method also uses amplification reactions using gene specific forward and reverse primers that are targeted to the hairpin sequence of pri- and pre-microRNA precursors to detect the expression levels of both the pri- and the pre-microRNAs.

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Patent Owner(s)

Patent OwnerAddress
THE OHIO STATE UNIVERSITY RESEARCH FOUNDATION1216 KINNEAR ROAD COLUMBUS OH 43212

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Schmittgen, Thomas D Granville, US 7 56

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