Method of obtaining idolocarbazoles using biosynthetic rebeccamycin genes
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United States of America Patent
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Jun 26, 2012
Grant Date -
Jan 3, 2008
app pub date -
Oct 17, 2002
filing date -
Oct 19, 2001
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Abstract
Method for production of indolocarbazoles by using rebeccamycin biosynthetic genes. The invention is based on utilization of rebeccamycin biosynthetic genes from Saccharothrix aerocolonigenes for production of indolocarbazoles in related microorganisms (Streptomyces spp.). Method includes the isolation of a DNA fragment from Saccharothrix aerocolonigenes ATCC39243 containing the rebeccamycin biosynthesis gene cluster and the expression of these genes in Streptomyces albus, achieving production of rebeccamycin and derivatives. The invention is suitable for use in the pharmaceutical field.
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