Protease screening methods and proteases identified thereby
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United States of America Patent
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Jul 3, 2012
Grant Date -
May 14, 2009
app pub date -
Jul 5, 2007
filing date -
Jul 5, 2006
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Abstract
Methods for identifying modified proteases with modified substrate specificity or other properties are provided. The methods screen candidate and modified proteases by contacting them with a substrate, such as a serpin, an alpha macroglobulins or a p35 family protein or modified serpins and modified p35 family members or modified alpha macroglobulins, that, upon cleavage of the substrate, traps the protease by forming a stable complex. Also provided are modified proteases.
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