Methods of optimal purification of nucleic acids and kit for use in performing such methods
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United States of America Patent
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Jul 17, 2012
Grant Date -
Mar 3, 2011
app pub date -
Aug 28, 2009
filing date -
Aug 28, 2009
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Abstract
A method and kit which allow the use of a discrete amount of a binding matrix to first purify nucleic acids from a medium under a first set of binding conditions wherein the amount of nucleic acid bound to the binding matrix is essentially independent of the amount of surface area of the definable amount of the binding matrix, followed by a second purification step wherein the nucleic acids are bound to a discrete amount of binding matrix under a second set of binding conditions wherein the amount of nucleic acid bound to the binding matrix is essentially dependent on the amount of surface area of the definable amount of the binding matrix, thus providing a discrete quantity of nucleic acid.
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