Optimization of crossover points for directed evolution
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United States of America Patent
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Jul 17, 2012
Grant Date -
Jan 24, 2008
app pub date -
Jun 12, 2007
filing date -
Mar 9, 2002
priority date (Note) -
Expired
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Abstract
Methods and devices for more efficiently engineering diversity into recombinant polypeptides and/or nucleic acids are provided herein. For example, a variety of methods of selecting and/or assessing potential crossover sites in an amino acid sequence or a nucleotide sequence are provided, as well as the resulting chimeric product sequences. These methods include, e.g., consideration of structural, functional and/or statistical data in the selection and assessment of sequences and crossover sites for use in recombination.
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