Method and apparatus for facilitating evaluating migration of cells in vitro
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United States of America Patent
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Jul 24, 2012
Grant Date -
Jul 29, 2010
app pub date -
Jan 27, 2009
filing date -
Jan 27, 2009
priority date (Note) -
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Abstract
Method and apparatus for processing a cell culture are provided. The method includes establishing a cell culture within a holding device having one or more wells, each well holding a cell culture, and including a well substrate with at least one electrode in contact with the cell culture; periodically applying at least one electrical pulse to the at least one electrode to prevent cells from attaching to and achieving confluence over the at least one electrode while allowing cells to attach to and achieve confluence over other portions of the well substrate; and discontinuing the periodically applying of the at least one electrical pulse to the at least one electrode after cells have achieved confluence over the other portions of the well substrate, and thereafter, monitoring the cell culture to monitor migration of cells over the electrode(s) from the other portions of the well substrate.
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