Process and equipment for plasmid purification
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United States of America Patent
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Aug 7, 2012
Grant Date -
May 18, 2006
app pub date -
Jan 9, 2006
filing date -
Jul 19, 1996
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Abstract
A scalable alkaline lysis process, including procedures and devices for the isolation of large quantities (grams and kilograms) of plasmid DNA from recombinant E. coli cells. Effective, controllable, and economical operation, and consistent low level of host chromosomal DNA in the final plasmid product. Involves a series of new unit operations and devices for cell resuspension, cell lysis, and neutralization.
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