Method for pairwise sequencing of target polynucleotides
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United States of America Patent
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Aug 7, 2012
Grant Date -
Apr 19, 2012
app pub date -
Dec 19, 2011
filing date -
Oct 6, 2006
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Abstract
The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.
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