Determination of single peak melting temperature by PCR analogy and double sigmoid equation
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United States of America Patent
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Sep 11, 2012
Grant Date -
Oct 21, 2010
app pub date -
Apr 17, 2009
filing date -
Apr 17, 2009
priority date (Note) -
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Abstract
Systems and methods for determining melting temperatures, Tm, for DNA from melt curve data. The systems and methods also allow for quantitative determination of gene amount based on peak height. A PCR analogy is used to perform quantization of an acquired melting curve dataset. The melting curve is transformed using a horizontal flip and a horizontal translation, and a double sigmoid equation is then fit to the data. Inverse translation and inverse horizontal flip transforms are applied to the equation to produce an equation based solution of the melt curve dataset. The equation based solution of the melt curve is then used to determine the first derivative (e.g., Tm value) and peak height.
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