Cloning, expression and purification of recombinant porcine intrinsic factor for use in diagnostic assay

Number of patents in Portfolio can not be more than 2000

United States of America Patent

PATENT NO 8288124
APP PUB NO 20100124754A1
SERIAL NO

12275102

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The present invention relates to a new method of producing functionally active recombinant porcine Intrinsic Factor as well as the protein (i.e., porcine Intrinsic Factor) produced thereby. In particular, the vector comprising the DNA encoding the protein is introduced into a Chinese Hamster Ovary (CHO) host cell under conditions sufficient for optimal expression of functional protein. The expressed protein may then be subjected to three methods ultimately resulting in a protein having at least 97% purity.

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Patent Owner(s)

Patent OwnerAddress
ABBOTT LABORATORIES100 ABBOTT PARK ROAD PATENT & TRADEMARK DEPT D377 AP6A-1 ABBOTT PARK IL 60064

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Leary, Thomas P Kenosha, US 24 161
Pan, You Libertyville, US 13 7
Uher, John F Kenosha, US 3 4
Wonderling, Ramani S Ivanhoe, US 17 34

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