Cloning, expression and purification of recombinant porcine intrinsic factor for use in diagnostic assay

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United States of America Patent

PATENT NO 8288124
APP PUB NO 20100124754A1
SERIAL NO

12275102

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Abstract

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The present invention relates to a new method of producing functionally active recombinant porcine Intrinsic Factor as well as the protein (i.e., porcine Intrinsic Factor) produced thereby. In particular, the vector comprising the DNA encoding the protein is introduced into a Chinese Hamster Ovary (CHO) host cell under conditions sufficient for optimal expression of functional protein. The expressed protein may then be subjected to three methods ultimately resulting in a protein having at least 97% purity.

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Patent Owner(s)

  • ABBOTT LABORATORIES

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Leary, Thomas P Kenosha, US 23 156
Pan, You Libertyville, US 10 6
Uher, John F Kenosha, US 3 4
Wonderling, Ramani S Ivanhoe, US 17 31

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