Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis
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United States of America Patent
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Nov 20, 2012
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app pub date -
Dec 29, 2006
filing date -
Dec 1, 2000
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Abstract
Nucleotide triphosphate probes containing a molecular and/or atomic tag on a γ and/or β phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.
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