from wood chips
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United States of America Patent
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Nov 27, 2012
Grant Date -
Jun 30, 2011
app pub date -
Oct 23, 2008
filing date -
May 7, 2008
priority date (Note) -
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Abstract
To extract and detect Bursaphelenchus xylophilus DNA without isolating Bursaphelenchus xylophilus from a piece of wood taken from a tree belonging to the genus Pinus and the like, provided are a method for extracting Bursaphelenchus xylophilus DNA from a collected piece of wood that includes Bursaphelenchus xylophilus, a LAMP primer set including primers that anneal to a specific region of Bursaphelenchus xylophilus DNA, and a method for detecting Bursaphelenchus xylophilus from a piece of wood by amplifying a DNA by a LAMP method using this primer set.
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