Methods and means for obtaining modified phenotypes
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United States of America Patent
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Dec 18, 2012
Grant Date -
Oct 25, 2012
app pub date -
May 17, 2012
filing date -
Aug 13, 1999
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Abstract
Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated target-specific RNA is provided by transcription of a chimeric gene comprising a promoter, a DNA region encoding the target-specific RNA, a self-splicing ribozyme and a DNA region involved in 3′ end formation and polyadenylation.
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