Method for creating intracellular artificial nanostructures in situ
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United States of America Patent
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Dec 25, 2012
Grant Date -
Apr 15, 2010
app pub date -
Aug 20, 2008
filing date -
Aug 8, 2005
priority date (Note) -
Expired
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Abstract
A method of creating intracellular artificial nanostructures in situ, which employees a chemical precursor. The precursor does not self-assemble due to the presence of a cleavable motif linked to it. When the precursor comes inside live cells by an uptaking mechanism on the cell membrane, the cleavable motif is then to be removed by an enzymatic action of a first enzyme. Without the cleavable motif, the precursor now engages in a self-assembling process to form nanostructures within the live cells, which may cause formation of a hydrogel. Furthermore, the self-assembling process can be made reversible by employing a second enzyme which puts the cleavable motif back to the precursor, whereby dissolving the nanostructures into solution.
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