Rationally-designed meganuclease variants of lig-34 and I-crei for maize genome engineering
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United States of America Patent
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Dec 25, 2012
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May 12, 2011
app pub date -
Sep 10, 2010
filing date -
Mar 11, 2008
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Abstract
The invention relates to the field of molecular biology and recombinant nucleic acid technology. In particular, the invention relates to a rationally-designed, non-naturally-occurring meganuclease with altered DNA recognition sequence specificity which recognizes and cleaves a unique DNA site in the maize genome. Disclosed herein are meganucleases which are variants of the I-CreI and LIG-34meganucleases. The invention also relates to methods of producing engineered maize plants using such meganucleases.
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