Integrated instrument performing synthesis and amplification, and a system and method thereof
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United States of America Patent
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Jan 22, 2013
Grant Date -
Jul 21, 2011
app pub date -
Jul 22, 2010
filing date -
Jan 23, 2008
priority date (Note) -
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Abstract
An integrated instrument for oligonucleotide synthesis and PCR, and a system and method thereof are disclosed. The integrated instrument is basically composed of two independent modules. The first module is a unit for chemical de novo synthesis of oligonucleotides such as oligonucleotide primers and/or oligonucleotide hybridization probes. The second module is a unit for performing an analytical polymerase chain reaction amplification in real time, i.e. a qPCR. The two modules are operatively linked to each other in such a way that a user can load a nucleic sample to be analyzed into the integrated instrument and perform a PCR reaction by programming the instrument without a previous external synthesis of oligonucleotide amplification primers.
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