3D imaging of live cells with ultraviolet radiation
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United States of America Patent
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Feb 5, 2013
Grant Date -
Jun 14, 2012
app pub date -
Feb 22, 2012
filing date -
Feb 18, 2008
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Abstract
A method for 3D imaging of cells in an optical tomography system includes moving a biological object relatively to a microscope objective to present varying angles of view. The biological object is illuminated with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm. Radiation transmitted through the biological object and the microscope objective is sensed with a camera from a plurality of differing view angles. A plurality of pseudoprojections of the biological object from the sensed radiation is formed and the plurality of pseudoprojections is reconstructed to form a 3D image of the cell.
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