Position dependent recognition of GNN nucleotide triplets by zinc fingers
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United States of America Patent
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Feb 26, 2013
Grant Date -
Oct 2, 2008
app pub date -
Aug 15, 2007
filing date -
Mar 24, 1999
priority date (Note) -
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Abstract
The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5′-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity.
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