Cyanine compounds and their use in staining biological samples
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United States of America Patent
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Feb 26, 2013
Grant Date -
May 6, 2010
app pub date -
Oct 28, 2009
filing date -
Oct 31, 2008
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Abstract
Cyanine compounds having the general formula I for staining biological samples, wherein R1, R2, X, Y, A1 and A2 are as defined in the specification. These kinds of compounds may show good light illumination stability, have a maximum absorption peak around 640 nm that may not change as a function of ambient temperature, have rapidly increased fluorescence intensity upon binding to nucleic acids to form compound/nucleic acid complexes, and have a light spectrum in the near-infrared region, thereby effectively reducing interference from background fluorescence and increasing the accuracy of the detection when used as a staining agent for nucleic acids in a flow cytometer. The compounds provided can be used as a staining agent for erythroblasts in the blood.
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