Polymerase chain reaction method, polymerase chain reaction droplet device, and polymerase chain reaction droplet device array
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United States of America Patent
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Mar 5, 2013
Grant Date -
Jun 30, 2011
app pub date -
Feb 10, 2010
filing date -
Dec 29, 2009
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Abstract
The present invention discloses a polymerase chain reaction (PCR) method, a PCR droplet device and a PCR droplet device array. The steps of the method comprise that a liquid comprising an analyzer is dropped on the heating coil disposed on the droplet device to form a droplet, then dropping a hydrophobic solution to prevent the droplet from evaporating. When an electric current or a voltage is supplied through at least one conducting wire to heat the heating coil, the inside of the droplet can generate buoyancy to drive the analyzer to move to the top of the inside of the droplet. Subsequently, the analyzer is moved to a periphery of the inside of the droplet so as to form a thermal cycle. Therefore the template is amplified by recycling the thermal cycle.
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