Purification of factor VIII using a mixed-mode or multimodal resin
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United States of America Patent
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Mar 19, 2013
Grant Date -
Aug 12, 2010
app pub date -
Jul 11, 2008
filing date -
Jul 11, 2007
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Abstract
A method for purifying a recombinant protein using a multimodal or mixed mode resin containing ligands which comprise a hydrophobic part and a negatively charged part is described. The invention is advantageous in that it is a single step chromatographic process which does not require adjustment of pH or conductivity during loading step and results in high yield and potency. The process is used for the purification of recombinant compositions of coagulation factor, particularly recombinant Factor VIII.
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