Methods for the generation of cartilage-like material by mechanical loading
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United States of America Patent
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Apr 9, 2013
Grant Date -
Jun 11, 2009
app pub date -
Dec 6, 2007
filing date -
Dec 6, 2006
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Abstract
A cartilage-like biomaterial is bioengineered by using a self-aggregating suspension cell culture with hydrostatic mechanical force without the use of a scaffold or foreign matrix for cell attachment during culture. The cells in suspension culture may be preconditioned prior to application of the hydrostatic mechanical force, such as hydrostatic pressure, for a period of time in the range of about 1 week to about 10 weeks. The cartilage-like biomaterial shares critical structural, phenotype, and functional characteristics with native, intact cartilage tissue.
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