Noninvasive method for measuring histamine from skin as an objective measurement of itch
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United States of America Patent
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Apr 16, 2013
Grant Date -
Mar 24, 2011
app pub date -
Sep 18, 2009
filing date -
Sep 18, 2009
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Abstract
A method for measuring of histamine in an epidermis comprising applying an adhesive article to an epithelium of a mammal; allowing for adherence of epithelial cells to the adhesive article; removing the adhesive article from the epithelium of the mammal; preparing the adhesive article using standard laboratory methods for extraction; extracting histamine from the epithelial cells adhered to said adhesive article; measuring histamine from the epithelial cells adhered to said adhesive article; determining the amount of histamine in the epithelial cells as compared to a baseline sample. Further, a method of objectively measuring the perception of itch in mammals and wherein there is a reduction in histamine from a baseline level which is directly proportional to a reduction in an itch perception.
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