Identification of immunoglobulin (lg) disorders using fourier transform infrared spectroscopy
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United States of America Patent
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Apr 16, 2013
Grant Date -
Jan 21, 2010
app pub date -
Jul 17, 2008
filing date -
Jul 17, 2008
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Abstract
A method and a corresponding system for obtaining a serum mid-infrared spectroscopic profile using Fourier-transform infrared spectroscopy (FTIR) are described. The method comprises acquiring FTIR spectra for dried sera and preprocessing the FTIR spectra of sera by differentiation and smoothing to enhance weak spectral features and to remove baseline variations. The preprocessed FTIR spectra are normalized to a common intensity range, the normalization being performed in a spectral sub-region defined by strongest infrared (IR) absorption for a protein to obtain the serum spectroscopic profile. The serum spectroscopic profiles provide a basis to diagnose immunoglobulin disorders or to quantify serum immunoglobulin levels.
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