Methods for rapid multiplexed amplification of target nucleic acids
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United States of America Patent
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Apr 23, 2013
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Apr 4, 2008
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Apr 4, 2007
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Abstract
A fast, multiplexed PCR system is described that can rapidly generate amplified nucleic acid products, for example, a full STR profile, from a target nucleic acid. Such systems include, for example, microfluidic biochips and a custom built thermal cycler, which are also described. The resulting STR profiles can satisfy forensic guidelines for signal strength, inter-loci peak height balance, heterozygous peak height ratio, incomplete non-template nucleotide addition, and stutter.
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