Detection of sub-cellular compartment localization of a molecule using a reduced affinity enzyme complementation reporter system
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United States of America Patent
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Apr 23, 2013
Grant Date -
Nov 11, 2010
app pub date -
Dec 26, 2007
filing date -
Dec 26, 2006
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Abstract
Methods and compositions for detecting the sub-cellular localization of a molecule are provided. Aspects of the invention include detecting translocation of a cell-surface receptor to a sub-cellular compartment, e.g., the endosome, using a reduced affinity enzyme complementation reporter system. Also provided are systems and kits for use in practicing embodiments of the methods.
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