Basic protein purification tags from thermophilic bacteria
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United States of America Patent
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Apr 23, 2013
Grant Date -
Apr 5, 2012
app pub date -
Dec 1, 2011
filing date -
Apr 15, 2005
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Abstract
The invention is related to a method for purification of recombinant proteins using highly basic proteins from thermophilic bacteria as purification tags for use in a cation-exchange chromatography purification step. The basic proteins may be ribosomal proteins. The recombinant proteins are expressed in eukaryotic or prokaryotic host cells. The purification tag will typically have a pl above about 9 and comprise from about 15 to about 250 amino acid residues.
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