Fluorescence resonance energy transfer assays for sarco/endoplasmic reticulum calcium atpase and phospholamban
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United States of America Patent
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Apr 30, 2013
Grant Date -
Jan 26, 2012
app pub date -
Jan 21, 2010
filing date -
Jan 22, 2009
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Abstract
Provided herein are methods for identifying molecules capable of modulating SERCA, the SERCA-PLB complex or the microenvironment of the complex. An exemplary assay provided herein is fluorescence resonance energy transfer (FRET). Also provided herein are FRET assays that are optimized for high-throughput screening (HTS) for identifying small molecules that modulate SERCA or the SERCA-PLB complex. Further provided are kits for carrying out said methods for identifying molecules.
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