Polypeptides and biosynthetic pathways for the production of monatin and its precursors
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United States of America Patent
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May 7, 2013
Grant Date -
May 7, 2009
app pub date -
May 20, 2008
filing date -
Apr 23, 2002
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Abstract
Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.
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