Method of detecting colon cancer marker

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United States of America Patent

PATENT NO 8445202
APP PUB NO 20100323367A1
SERIAL NO

12875207

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Abstract

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It is intended to provide a non-invasive and convenient method of detecting a tumor marker for diagnosing colon cancer which is superior in sensitivity and specificity to the existing fecal occult blood test. More specifically speaking, a method of detecting a tumor marker for diagnosing colon cancer which comprises collecting biological sample which is immediately frozen using liquid nitrogen in some cases, homogenizing the sample in the presence of an inhibitor of an RNA digesting enzyme to give a suspension, extracting RNA from the obtained suspension, subjecting the extracted RNA to reverse transcription to give cDNA. amplifying the obtained cDNA and then detecting the thus amplified cDNA. This method is characterized by involving no procedure of separating cell components from the biological sample.

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Patent Owner(s)

Patent OwnerAddress
NATIONAL UNIVERSITY CORPORATION HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE20-1 HANDAYAMA 1 CHOME HIGASHI-KU HAMAMATSU-SHI SHIZUOKA 431-3192

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kanaoka, Shigeru Hamamatsu, JP 7 15

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