O157:H7

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United States of America Patent

PATENT NO 8445209
APP PUB NO 20100311062A1
SERIAL NO

12780707

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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Disclosed are primer and probe compositions, PCR assays, methods and kits for the specific detection of E. coli O157:H7 and not E. coli O55:H7 from a variety of samples. In some embodiments, methods for specifically detecting E. coli O157:H7 comprise: hybridizing at least a first pair of polynucleotide primers to at least a first target polynucleotide sequence, hybridizing at least a second pair of polynucleotide primers to at least a second target polynucleotide sequence, amplifying said at least first and said at least second target polynucleotide sequences, and detecting said at least first and said at least second amplified target polynucleotide sequence products, wherein the detection of both the first amplified target polynucleotide sequence product and the second amplified target polynucleotide sequence product is indicative of the presence of E. coli O157:H7 in a sample and not E. coli O55:H7.

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Patent Owner(s)

Patent OwnerAddress
LIFE TECHNOLOGIES CORPORATION5791 VAN ALLEN WAY CARLSBAD CA 92008

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Barker, Melissa Gilroy, US 4 9
Brzoska, Pius Woodside, US 38 197
Cummings, Craig A Pacifica, US 1 2
Furtado, Manohar San Ramon, US 35 100
Petrauskene, Olga San Carlos, US 14 29
Vatta, Paolo San Mateo, US 18 86
Wong, Lily Oakland, US 11 44

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