Cell-free system for synthesis of proteins derived from cultured mammalian cells
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United States of America Patent
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May 21, 2013
Grant Date -
Dec 8, 2011
app pub date -
Aug 16, 2011
filing date -
Nov 17, 2004
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Abstract
Prepared is an extract composition having an improved protein synthetic activity in a cell-free protein synthesis system using a mammalian cultured cell extract. An eukaryotic translation initiation factor and/or translational regulator are added to a cell-free protein synthesis system comprising an extract prepared from cultured mammalian cells and a template mRNA. These factors are one or more selected from the group consisting of eukaryotic translation initiation factors 4E (eIF4E), 2 (eIF2) and 2B (eIF2B), and eukaryotic translational regulator p97.
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