Photobleaching and intermittency localization microscopy
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United States of America Patent
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May 21, 2013
Grant Date -
Dec 13, 2012
app pub date -
Feb 22, 2011
filing date -
Feb 23, 2010
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Abstract
A method of image analysis creates super-resolution images from images with high densities of fluorophores by processing a movie in which the fluorescent molecules or particles are photobleaching or blinking. The method looks for the individual photobleaching events that can be located with high resolution (nm to tens of nanometers scale). The positions of the photobleaching or blinking events are then drawn in a composite image that is at a much higher resolution than the original fluorescence movie.
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