Method and apparatus for generating and positioning micro-scale evanescent fields
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United States of America Patent
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May 28, 2013
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app pub date -
Oct 16, 2009
filing date -
Oct 16, 2008
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Abstract
Illumination sources are connected to ends of micropipette probes having total internal reflection tips. The total internal reflection produces evanescence at the tips. The evanescence causes fluorescence of dye marked target molecules at tips of the probes. The evanescence is limited to about 100 nm from the tips. The presence of a target molecule at the end of a probe is observed by observing the fluorescence. The limited penetration of the evanescence eliminates noise in the signal and unwanted bleaching of dyes in the cell, so that repeated, multiple and arrays of probes may be applied.
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